NextGen sequence analysis of two sex-linked P. tremuloides genomic regions on chromosome 19
نویسندگان
چکیده
Material and methods Using DNA probes derived from these SSR markers, two BAC clones were isolated from a BAC library of the P. tremuloides male clone (Pakull B. et al., 2011, Can. J. For. Res. 41, 245-253) and further analyzed by454 sequencing (GATC Biotech AG). The generated single end reads were assembled to contigs using Newbler and Mira. The set of combined contigs (Newbler and Mira) of each BAC was subjected to scaffolding by SeqMan Pro (DNASTAR Lasergene) together with the related BAC end sequences created by Sanger sequencing (Pakull B. et al., 2011, Can. J. For. Res. 41, 245-253). The subsequent combination of the created scaffolds to BAC consensus sequences was assisted by Sanger sequencing of PCR amplified scaffold ends and connections.
منابع مشابه
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a 5 # Equal contribution 6 Departments of a Botany, 19 Supplementary information: All supplementary files will be made available upon request. 20 Data Accessibility: All sequence data will be deposited on Genbank and SRA and all 21 other data files on Dryad. 22 23 peer-reviewed) is the author/funder. All rights reserved. No reuse allowed without permission. Abstract 24 All species of the genus ...
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